Bioreactor Sparger

I-bioreactors encinci inokusasaza ngokufanelekileyo ioksijini kwaye isuse i-carbon dioxide ngaphandle kwe-nebulizers.Nangona kunjalo, la manyathelo awasebenzi kwii-bioreactors ezinkulu, njengoko indawo engaphantsi komhlaba ukuya kwi-volume ratio ikhokelela ekuqokeleleni kwe-carbon dioxide kwaye inqanda ukungena kwe-oksijini.Ngoko ke, i-nebulizers iyimfuneko ekufakweni kwe-oksijini kunye nokususwa kwe-carbon dioxide.

Iinkqubo ezine-nebulizers ezincinci kunye ezinkulu zihlala ziluncedo kuba zinokuhlangabezana neemfuno zenkqubo ezahlukeneyo.Ngokomzekelo, ii-nebulizers ezinkulu zivelisa amaqamza amakhulu asusa ngokufanelekileyo i-CO 2 kwisisombululo, kodwa amaqamza amakhulu afuna ukuphazamiseka okunamandla ukuze awaphule kwaye akhulule ioksijini.

Ngelixa oku kunokusebenza kakuhle kwimigca yeeseli ekwazi ukumelana nokubanda, ukushukumisa kungonakalisa iiseli ezibuthathaka ezincancisayo.Kule meko, i-macro-distributor ephantsi ingasetyenziselwa kuqala ukususa i-CO 2 kwaye emva koko i-micro-distributor kwi-series ukuvelisa ama-bubbles amancinci ahambisa i-oksijini ngokufanelekileyo.

Umngeni: Iimpawu zeBubble zimisela i-O2 yezoThutho kunye ne-CO 2 ye-Vapor Extraction Rates

Ukubunjwa kwebhamuza kunye nobukhulu buchaphazela kakhulu indlela ioksijini esasazeka ngayo kuyo yonke i-bioreactor.Iimpawu zeBubble ziphenjelelwa kakhulu bubungakanani bepore kunye nokuhanjiswa, imathiriyeli yokuhambisa, izinga lokuhamba, iipropathi zolwelo kunye negesi, kunye noxinzelelo.Umzekelo, izitshizi ezincinci zivelisa amaqamza amancinci, angqukuva, ngelixa izitshizi ezikhulu zivelisa amaqamza amakhudlwana nangaphantsi ngokufanayo.

Ii-micro spagers zivelisa ubungakanani be-micron kunye namaqamza angqukuva, kwaye ukuxinezeleka komphezulu yeyona nto iphambili njengoko zidlula kwi-bioreactor.Ngoko ke, banexesha elide lokuhlala kwi-reactor, ephucula ukuhanjiswa kwe-oksijeni, kodwa ayifanelekanga ukususa i-carbon dioxide kwinkcubeko.

I-nebulizers ezinkulu zivelisa amaqamza kunye nobubanzi obuyi-1-4 mm, apho ukuxinana komphezulu kunye ne-buoyancy kwi-broth idibanisa ukuchaphazela ukumila kunye nokuhamba.La maqamza anexesha elifutshane lokuhlala kodwa anqabile ukuba anyibilike kunamaqamza amancinci.Nangona kunjalo, i-micro spagers inokuvelisa amaqamza amakhulu e-asymmetric, kunye namandla angaphakathi alawula ukuziphatha kwabo.La maqamza anokudubula ngokulula ngaphandle kokunyibilika okanye ukukhulula i-CO2.

Ubume kunye nobukhulu bamaqamza bumisela ubungakanani boxinzelelo lwe-shear iseli iya kuba nayo, ukusebenza kakuhle kokuhlutha i-CO 2 kwinkqubo, kunye nezinga lokudluliselwa kwe-oksijini kwiseli.Ngoko ke, kubalulekile ukwandisa i-nebulizer ye-bioreactor ukuqinisekisa ukuba amaqamza e-oksijini afanayo ngobukhulu kunye nokusabalalisa kwaye angonakalisi iiseli.

Isisombululo: Sebenzisa Ukulawulwa koMgangatho weMveliso engqongqo ye-HENGKO Bioreactor Sparger

I-HENGKO ineminyaka engaphezu kwamashumi amabini yamava okuphuhlisa kunye nokuvelisa i-sintered sparger.I-sparger yethu yensimbi engenasici ziziphumo zeenjineli ezininzi ezihlala ziphucula indlela yokuvelisa ukuvelisa le mveliso ikumgangatho ophezulu kunye neepores ezifanayo kwaye, ngoko ke, ubungakanani obufanayo bebhamu bukhutshelwe kwi-bioreactor.I-sparger yethu ene-porous inconywa ukuba isetyenziswe kunye nabalawuli bokuhamba kobunzima obuphantsi.

Kusetyenziswa kwanjani:Isilawuli sokuhamba kobunzima obuphantsi singenisa ioksijini kancinane kwiporous sparger.I-sparger ayikhupheli igesi ngokukhawuleza.Endaweni yoko, uxinzelelo luyanda ngokuthe ngcembe de kufikelelwe kwindawo ebaluleke kakhulu, apho amaqamu akhutshwa ngobunono kwi-bioreactor.

Ukusebenzisa le ndlela ye-sparging, i-oxygen mass flow rate ingatshintshwa ukulawula izinga lokukhululwa kweebhubhu kwi-bioreactor.Imingxuma kwi-sparger mincinci ngokwaneleyo ukuba amaqamza ayakwenzeka ngokuqikelelwayo.Ke ngoko, le teknoloji ye-bioreactor sparging iyancipha kuzo zonke iinqanawa zobukhulu, kunye nesantya sokuhambisa i-oksijini ngokomlinganiselo wokuhamba kwerhasi.

Imibuzo malunga neBioreactor Sparger

1. Yintoni iSparger kwi-Bioreactor?

Ngamafutshane, i-sparger sisixhobo esisetyenziswa kwi-bioreactor ukwazisa iigesi, ezinjengeoksijini okanye umoya, kwindawo yolwelo.Umsebenzi oyintloko we-sparger kukubonelela ngeoksijini kwi-microorganisms kwi-bioreactor, eyimfuneko ekukhuleni kwabo kunye ne-metabolism.

I-Sparger kwi-bioreactor isetyenziselwa ukongeza iigesi ezifana ne-oxygen, umoya, okanye ezinye iigesi ezifunekayo ekukhuleni kunye ne-metabolism ye-microorganisms.I-oksijeni inikezelwa kwii-microorganisms ngokusebenzisa i-Sparger, enceda ukugcina amanqanaba e-oksijini echithwe kwi-medium medium.Amanqanaba e-oksijeni echithwe yiparameter ebalulekileyo yokubeka iliso kunye nokulawula ngexesha le-bioprocess njengoko ichaphazela ngokuthe ngqo ukukhula kunye ne-metabolism ye-microorganisms.

ISparger yenzelwe ukwazisa irhasi kulwelo oluphakathi ngendlela elawulwayo, njengokusebenzisa izinto ezirhabaxa okanye iityhubhu.I-Sparger inokufumaneka ngezantsi okanye phezulu kwi-bioreactor, kuxhomekeke kuyilo lwe-bioreactor kunye nohlobo lwee-microorganisms ezisetyenzisiweyo.I-Sparger inokulungelelaniswa ukuze inikeze izinga lokudluliselwa kwe-oksijini elifunwayo kunye nokugcina inqanaba elifanelekileyo le-oksijini elichithiweyo phakathi.

I-Sparger nayo idlala indima ebalulekileyo ekugcineni izinga lokudluliselwa kobuninzi, okuyinqanaba apho i-oksijini idluliselwa kwisigaba segesi ukuya kwisigaba samanzi.Izinga lokudlulisa ubuninzi linokuchatshazelwa yizinto ezifana nobukhulu kunye nokuma kwe-bioreactor, uhlobo kunye noxinzelelo lwe-microorganisms, kunye nobushushu kunye ne-pH ye-medium.I-Sparger ingasetyenziselwa ukulawula ezi zinto kunye nokwandisa izinga lokudluliselwa ngobuninzi, okubalulekileyo kwimpumelelo ye-bioprocess.

Isishwankathelo, owona msebenzi uphambili we-sparger kwi-bioreactor kukubonelela ngeoksijini kwi-microorganisms kwindawo yolwelo, eyimfuneko ekukhuleni kwabo kunye nemetabolism, kunye nokugcina amanqanaba afanelekileyo e-oksijini anyibilikisiweyo kunye nenqanaba lokudluliselwa kobuninzi, eziyimfuneko impumelelo ye-bioprocess.

Lithetha ukuthini igama elithi Sparger?

ISparger sisixhobo esisetyenziselwa ukungenisa igesi kulwelo.Isetyenziswa ngokuqhelekileyo kwii-bioreactors, eziziinqanawa ezikhethekileyo ezisetyenziselwa ukuhlakulela i-microorganisms okanye iiseli phantsi kweemeko ezilawulwayo.

Yintoni umsebenzi weSparger?

Umsebenzi we-sparger kukubonelela nge-oksijeni okanye enye igesi kwi-bioreactor ukwenzela ukuxhasa ukukhula kunye ne-metabolism yeeseli okanye i-microorganisms.

Yintoni ukusetyenziswa kweSparger kwi-Bioreactor Ukuchaza iintlobo zayo?

Kukho iintlobo ezininzi ze-sparger ezinokuthi zisetyenziswe kwi-bioreactor.Ezi ziquka i-bubble spagers, edala umjelo oqhubekayo wamaqamza kulwelo, kunye ne-spargers yokutshiza, esasaza igesi njengenkungu ecolekileyo.Ezinye iintlobo ze-sparger zibandakanya i-sparger e-porous kunye ne-hollow-fiber spagers.

Iphi iSparger efumaneka kwiBioreactor?

I-sparger ngokuqhelekileyo ibekwe ezantsi kwe-bioreactor, apho inokuxuba ngokufanelekileyo igesi kunye nolwelo.Kwiinkqubo ezinkulu zokuvutshelwa, i-bubble spagers isoloko isetyenziswa njengoko ilula kwaye ingabizi kakhulu ukuyisebenzisa.

Loluphi uhlobo lweSparger olusetyenziswa kakhulu kuMbizo oMkhulu?

Kwiinkqubo ezinkulu zokuvutshelwa, i-bubble spagers isoloko isetyenziswa njengoko ilula kwaye ingabizi kakhulu ukuyisebenzisa.I-bubble sparger iqukethe ityhubhu okanye umbhobho onemingxuma emincinci okanye iindawo zokubeka apho irhasi ingeniswa kulwelo.Irhasi iqukuqela kwimingxuma okanye kwiindawo zokubeka kwaye yenza umjelo oqhubekayo wamaqamza kulwelo.I-Bubble spargers iyasebenza ekuboneleleni umthamo omkhulu wegesi kwi-bioreactor kwaye inokulungelelaniswa lula ukulawula izinga lokuhamba kwegesi.Kwakhona kulula ukuzicoca nokuzilungisa.Nangona kunjalo, i-bubble spagers inokudala amaqamza amakhulu kakhulu anokuthi angasebenzi kakuhle ekuboneleleni indawo ephezulu yeeseli okanye ii-microorganisms ukuba zidibane nerhasi.Kwezinye iimeko, i-spray sparger okanye olunye uhlobo lwe-sparger lunokufaneleka ngakumbi kwinkqubo ethile yokuvutshelwa.

Yintoni ekufuneka uyazi malunga neSparger System?

Kukho izinto ezininzi omele uziqwalasele xa usebenzisa inkqubo ye-sparger kwi-bioreactor.Ezi ziquka:

1. Ulungelelwaniso:Kubalulekile ukulinganisa ngokufanelekileyo izinga lokuhamba kwerhasi efakwe kwi-bioreactor.Oku kuya kuqinisekisa ukuba inani elichanekileyo legesi linikezelwa kwiiseli okanye i-microorganisms kwaye i-oksijini yoxinaniso kwi-bioreactor ingaphakathi koluhlu olufunwayo.

2. Uxinzelelo lweoksijini:Ugxininiso lwe-oksijini kwi-bioreactor kufuneka lubekwe esweni rhoqo ukuze kuqinisekiswe ukuba luphakathi koluhlu olufunwayo kwiiseli okanye ii-microorganisms ezilinywayo.Ukuba i-oxygen concentration iphezulu kakhulu okanye iphantsi kakhulu, inokuchaphazela ukukhula kunye nemetabolism yeeseli okanye i-microorganisms.

3. Uthintelo losulelo:Kubalulekile ukuqinisekisa ukuba i-sparger kunye nendawo ejikelezileyo ihlanjululwe ngokufanelekileyo kwaye igcinwe ukukhusela ukungcoliswa kwe-bioreactor.Oku kunokubandakanya ukutshintshwa rhoqo kwezihluzo zegesi kunye nokucoca i-sparger kunye neendawo ezingqongileyo ngezibulali-ntsholongwane ezifanelekileyo.

4. Isantya sokuhamba kwerhasi:Isantya sokuhamba kwegesi kufuneka silungiswe njengoko kufunekayo ukuze kugcinwe i-oksijini efunwayo kwi-bioreactor.Umlinganiselo wokuhamba unokufuna ukwandiswa okanye ukuncitshiswa ngokusekelwe kwimfuno ye-oksijini yeeseli okanye i-microorganisms kunye nesantya sokusetyenziswa kwegesi.

5. Ugcino:Ukugcinwa rhoqo kwenkqubo ye-sparger kubalulekile ukuqinisekisa ukuba isebenza ngokufanelekileyo nangokufanelekileyo.Oku kunokuquka ukukhangela iindawo ezivuzayo, ukubuyisela iindawo ezigugileyo okanye ezonakeleyo, nokucoca ispager neendawo ezisingqongileyo njengoko kuyimfuneko.

2. Umsebenzi ophambili weSparger kwiBioreactor?

Umsebenzi oyintloko we-sparger kwi-bioreactor kukwazisa iigesi, ezifana ne-oxygen okanye umoya, kwi-medium medium.Kuyimfuneko ekukhuleni kunye nemetabolism ye-microorganisms kwi-bioreactor, njengoko zifuna i-oksijini yokuphefumula.I-Sparger inceda ukubonelela ngee-microorganisms nge-oksijini efunekayo kwaye igcine inqanaba le-oksijini efanelekileyo kwi-bioreactor ukuxhasa ukukhula kwabo kunye ne-metabolism, ebalulekileyo kwimpumelelo ye-bioprocess.

A: Ukwazisa ngeegesi:Umsebenzi oyintloko we-sparger kwi-bioreactor kukwazisa iigesi, ezifana ne-oxygen okanye umoya, kwi-medium medium.Inceda ukubonelela ngee-microorganisms ngeoksijini efunekayo yokukhula kunye nemetabolism.

B: Ukugcina amanqanaba eoksijini enyibilikileyo:I-Sparger inceda ukugcina amanqanaba e-oksijini enyibilikile kwindawo yolwelo.Ezi nqanaba ziyiparameter ebalulekileyo ukubeka iliso kunye nokulawula ngexesha le-bioprocess njengoko lichaphazela ngokuthe ngqo ukukhula kunye ne-metabolism ye-microorganisms.

C: Ukulawula izinga lokudluliselwa kwerhasi:I-Sparger yenzelwe ukwazisa igesi kwindawo yolwelo ngendlela elawulwayo.I-Sparger inokulungelelaniswa ukuze inikeze izinga lokudluliselwa kwe-oksijini elifunwayo kunye nokugcina inqanaba elifanelekileyo le-oksijini elichithiweyo phakathi.

D: Ukugcina izinga lokudluliselwa kobunzima:I-Sparger nayo idlala indima ebalulekileyo ekugcineni izinga lokudluliselwa kobuninzi, okuyinqanaba apho i-oksijini idluliselwa kwisigaba segesi ukuya kwisigaba samanzi.

E: Ukuphucula inkqubo yebhayoloji:I-Sparger ingasetyenziselwa ukulawula izinto ezifana nobukhulu kunye nokuma kwe-bioreactor, uhlobo, kunye noxinzelelo lwe-microorganisms, kunye nobushushu kunye ne-pH ye-medium, ukwandisa i-bioprocess.

F: Ukubonelela ngokuxuba:I-Spargers ikwanceda ukwenza umxube we-homogenous wolwelo kunye negesi ngokubonelela ngesenzo sokuxuba.Inceda ekuboneleleni ii-microorganisms ngendawo efanayo.

3. Iintlobo zeSparger kwi-bioreactor?

Iindidi ezininzi ze-sparger zibandakanya i-sparger yamatye e-porous eyenziwe ngesixhobo esinemingxuma efana ne-ceramic okanye isinyithi esine-sintered kunye ne-bubble column spagers, ezisebenzisa uthotho lweityhubhu okanye imilomo ukungenisa igesi kulwelo.

Iindidi ezininzi ze-sparger zinokusetyenziswa kwi-bioreactor, kuquka:

1. Iiporous stone spagers:Ezi zenziwe ngezinto ezinobungozi ezifana ne-ceramic okanye i-sintered metal kwaye zibekwe phantsi kwe-bioreactor.Banikezela indawo enkulu yendawo yokuhanjiswa kwegesi kwaye isetyenziswa ngokuqhelekileyo kwii-bioreactors ezincinci.

2. Ibhamuza kwikholamu spagers:Ezi zisebenzisa uthotho lweityhubhu okanye imilomo ukungenisa irhasi kulwelo.Ziyakwazi ukubekwa ezantsi okanye phezulu kwi-bioreactor kwaye ziqhele ukusetyenziswa kwii-bioreactors ezinkulu.

3. Iringi sparger:Ezi zibekwe emazantsi e-bioreactor kwaye zisebenzise isakhiwo esimile njengesangqa ukuvelisa amaqamza kunye nokubonelela ngokuhanjiswa kweoksijini.

4. I-Micro-bubble Sparger:Ezi zenzelwe ukuvelisa amaqamza amancinci abonelela ngokusebenza kakuhle kokuhanjiswa kweoksijini kwaye asetyenziswa ngokubanzi kwi-bioreactors enkulu, enoxinaniso oluphezulu.

5. I-Jet sparger:Ezi zisebenzisa isethi yemilomo ukungenisa irhasi kulwelo.Ziyakwazi ukufakwa ngezantsi okanye phezulu kwi-bioreactor kwaye zisetyenziswa ngokuqhelekileyo kwi-high-shear bioreactors.

6. Paddlewheel sparger:Olu hlobo lweSparger lusebenzisa ivili lokugwedla elijikelezayo ukwenza amaqamza kunye nokubonelela ngokuhanjiswa kweoksijini.Isetyenziswa ngokuqhelekileyo kwiinkqubo zokuvutshelwa.

Ezi zezinye zeentlobo ze-sparger ezisetyenziswa ngokuqhelekileyo kwi-bioreactors, kwaye ukhetho lwe-Sparger luxhomekeke kubukhulu, uhlobo, kunye noyilo lwe-bioreactor kunye ne-bioprocess ethile esetyenziswayo.

4. Indlela yokuSeta amaNqanaba eSparging kwi-Bioreactor?

Inqanaba le-sparing kwi-bioreactor lidla ngokusekwe kwimfuno ye-oksijini ye-microorganisms, isantya sokudluliselwa kwegesi, kunye nesantya sokuhamba kwegesi.Izinto ezinokuchaphazela inqanaba le-sparging ziquka ubungakanani kunye nokuma kwe-bioreactor, uhlobo kunye noxinzelelo lwe-microorganisms, kunye nobushushu kunye ne-pH ye-medium.

5. Indima kaSparger kwiBioreactor?

Indima ye-sparger kwi-bioreactor kukwazisa iigesi, ezifana ne-oxygen okanye umoya, kwindawo yolwelo ukuhlangabezana nemfuno ye-oxygen ye-microorganisms.Ibalulekile ekukhuleni nasekukhuleni kwe-metabolism ye-microorganisms kwaye, ekugqibeleni, kwimpumelelo ye-bioprocess.

I-Sparger inceda ukubonelela ngee-microorganisms nge-oksijini efunekayo yokukhula kunye ne-metabolism.Inceda ukugcina amanqanaba e-oksijini echithwe kwi-medium medium, okuyimilinganiselo ebalulekileyo ukubeka iliso kunye nokulawula ngexesha le-bioprocess njengoko zichaphazela ngokuthe ngqo ukukhula kunye ne-metabolism ye-microorganisms.

ISparger yenzelwe ukwazisa irhasi kulwelo oluphakathi ngendlela elawulwayo, njengokusebenzisa izinto ezirhabaxa okanye iityhubhu.I-Sparger inokufumaneka ngezantsi okanye phezulu kwi-bioreactor, kuxhomekeke kuyilo lwe-bioreactor kunye nohlobo lwee-microorganisms ezisetyenzisiweyo.I-Sparger inokulungelelaniswa ukuze inikeze izinga lokudluliselwa kwe-oksijini elifunwayo kunye nokugcina inqanaba elifanelekileyo le-oksijini elichithiweyo phakathi.

I-Sparger nayo idlala indima ebalulekileyo ekugcineni izinga lokudluliselwa kobuninzi, okuyinqanaba apho i-oksijini idluliselwa kwisigaba segesi ukuya kwisigaba samanzi.Izinga lokudlulisa ubuninzi linokuchatshazelwa yizinto ezifana nobukhulu kunye nokuma kwe-bioreactor, uhlobo kunye noxinzelelo lwe-microorganisms, kunye nobushushu kunye ne-pH ye-medium.I-Sparger ingasetyenziselwa ukulawula ezi zinto kunye nokwandisa izinga lokudluliselwa ngobuninzi, okubalulekileyo kwimpumelelo ye-bioprocess.

I-Sparger ikwanceda ukwenza umxube we-homogenous wolwelo kunye negesi ngokubonelela ngesenzo sokuxuba.Inceda ekuboneleleni ii-microorganisms ngendawo efanayo.

Isishwankathelo, indima ye-sparger kwi-bioreactor kukubonelela ngeoksijini kwii-microorganisms kwindawo yolwelo, eyimfuneko ekukhuleni kwabo kunye nemetabolism, kunye nokugcina amanqanaba afanelekileyo e-oksijini enyibilikisiweyo kunye nesantya sogqithiso oluninzi, oluyimfuneko ekukhuleni kwayo. impumelelo ye-bioprocess.Ikwanceda ukwenza umxube we-homogenous kwaye ibonelela ngesenzo sokuxuba kwi-medium liquid.

Loluphi uhlobo lweBioreactor Sparger onomdla wokulisebenzisa okanye uLungise?

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