Yonke into omawuyazi malunga noSparger kwiFermenter

Yintoni iSparger kwiFermenter?

Ngamafutshane, uASparger kwi FermentersisiXhobo esiSetyenziselwe ukwazisa uMoya okanye ezinye iiGesi kwiNqanawa yokuBilisa.

Ngokuqhelekileyo ngumbhobho onombhobho ophantsi kwenqanawa okanye kufuphi ne-impeller kwaye ivumela igesi ukuba ikhutshwe kulwelo ngokusebenzisa imingxuma emincinci okanye iijethi.

IzizathuI-sparger kukubonelela ngeoksijini kwiinkqubo ze-aerobic fermentation okanye ukuxuba i-carbon dioxide (co2) kwiinkqubo ze-anaerobic fermentation. i-sparger inceda ukugcina inqanaba elifanelekileyo le-oksijini echithiweyo kwindawo yenkcubeko, eyimfuneko ekukhuleni kunye ne-metabolism ye-microorganisms, njengegwele okanye ibhaktheriya.

Ngethuba lenkqubo yokuvuthwa, i-sparger ingasetyenziselwa ukulawula i-PH, ukushisa kunye nokuxuba iziqulatho zomkhumbi. isetyenziswa ngokuqhelekileyo kwi-bioprocessing ye-industrial-scale, apho umthamo omkhulu we-microorganisms ukhuliswa ngaxeshanye, ukuqinisekisa ukuvutshelwa okuhambelanayo kunye nokusebenzayo.

Kwintsimi yokuvutshelwa, i-spargers idlala indima ebalulekileyo ekudaleni indawo efanelekileyo yokukhula kwe-microbial. Ngamagama alula, isparger sisixhobo esisetyenziselwa ukungenisa igesi (ngokuqhelekileyo ioksijini okanye icarbon dioxide) kulwelo. Ngexesha lokuvutshelwa, i-spargers isoloko isetyenziselwa ukulawula ukuxinwa kwe-oksijeni echithwe, isondlo esibalulekileyo sokukhula kwe-microbial. Kule post yebhlog, siza kuxoxa ngokweenkcukacha ukuba yintoni isisasazo, sisebenza njani, kunye nokubaluleka kwaso kwinkqubo yokuvutshelwa.

Yintoni isitofu?

ISparger sisixhobo esisetyenziselwa ukungenisa igesi (edla ngokuba yioksijini okanye icarbon dioxide) kulwelo oluphakathi. Abasasazi badla ngokubandakanya i-gas inlet kunye ne-porous material ukusabalalisa igesi kulwelo. Iimathiriyeli zePorous zingenziwa kwizinto ezahlukeneyo ezibandakanya isinyithi, iiseramics okanye iipolymers.

I-Spargers isetyenziswa ngokuqhelekileyo ukulawula ukuxinana kweoksijini enyibilikisiweyo kwimidiya engamanzi ngexesha lokuvutshelwa. Oku kungenxa yokuba i-oksijeni iyona sondlo esiphambili sokukhula kwe-microbial kwaye ukufumaneka kwayo kuchaphazela kakhulu isantya kunye nokusebenza kakuhle kokuvutshelwa. Ukongeza, ootitshala banokusetyenziselwa ukulawula i-pH yendawo yokuvundisa ngokuzisa i-carbon dioxide okanye ezinye iigesi ezine-asidi.

Uhlobo lomthengisi:

Kukho iintlobo ezininzi zee-spreader eziqhelekileyo ezisetyenziswa kwishishini lokubilisa, nganye ineengenelo zayo kunye nokungalunganga. Ezona ndidi zixhaphakileyo zesisasazo zezi:

1. Abasasazi bePorous:

Aba bahambisi benziwe ngezinto ezirhabaxa ezifana neentsimbi ezitshisiweyo, iiseramikhi okanye iipolymers. Ngokuqhelekileyo zisetyenziswa kwishishini ngenxa yokusebenza kwazo okuphezulu kunye nokulula kokusetyenziswa.

2. Izitofu zebhabhula:

Ezi zitofu zingenisa igesi kwindawo yokuvubela ngendlela yamaqamza. Azisebenzi kakuhle kune spargers ezinamathambo, kodwa zihlala zisetyenziselwa ukubilisa okuncinci.

3. I-Membrane spagers:

Ezi spargers zisebenzisa inwebu yerhasi ekwaziyo ukungena ukungenisa irhasi kwindawo yokubila. Ziyasebenza, kodwa kunzima ukuzicoca nokugcina.

Ukubaluleka kweespager kwizibilisi:

I-Spargers idlala indima ephambili kwinkqubo yokuvutshelwa ngokulawula i-oksijini edibeneyo kunye ne-pH kwi-fermentation medium. Ukuxinwa kwe-oksijini echithwe yiparameter ebalulekileyo yokukhula kwe-microbial njengoko ichaphazela izinga kunye nokusebenza kakuhle kokuvutshelwa. Ngokulawula ukuxinwa kwe-oksijeni echithwe, i-spargers inokunceda ukuqinisekisa ukuba inkqubo yokuvutshelwa ilungiselelwe isivuno esiphezulu kunye nomgangatho.

Ukongeza ekulawuleni ukuxinana kweoksijini enyibilikisiweyo, ii-nebulizers zinokusetyenziselwa ukulawula i-pH yendawo yokuvundisa. Oku kubalulekile kuba i-pH ichaphazela kakhulu izinga kunye nokusebenza kakuhle kokukhula kwe-microbial. Ngokuzisa iigesi ze-asidi ezifana nekhabhon diokside, i-spargers inokunceda ukugcina uluhlu lwe-pH olufanelekileyo lokukhula kwe-microbial.

Izinto ekufuneka ziqwalaselwe xa ukhetha isisasazo:

Xa ukhetha i-sparger ye-fermenter, kufuneka kuqwalaselwe iinkalo ezininzi, kuquka uhlobo lwe-microorganisms ekhuliswayo, ubukhulu be-fermenter, kunye neemeko ezinqwenelekayo zokuvundiswa. Umzekelo, i-spargers ene-porous fermentation ifaneleka ngakumbi kwi-fermentation enkulu ngenxa yokusebenza kwayo okuphezulu, ngelixa i-spargers ye-air bubble inokulungela ngcono ukubiliswa kwezinto ezincinci. Ukhetho lwe-sparger lunokuxhomekeka kwiimfuno ezithile zenkqubo yokuvutshelwa, njenge-concentration efunekayo ye-oksijeni edibeneyo okanye uluhlu lwe-pH oluphezulu.

ULondolozo lweSasazi kunye nokuCoca:

Ukugcinwa okufanelekileyo kwe-sparger kunye nokucoca kubalulekile ukuqinisekisa ukusebenza kakuhle kunye nokuthintela ungcoliseko. I-Sparger kufuneka icocwe ngamaxesha athile ukuze isuse nayiphi na i-buildup of microbial growth okanye ezinye izinto ezingcolisayo ezinokuchaphazela ukusebenza kwazo kakuhle. Inkqubo yokucoca inokuthi ihluke ngokuxhomekeka kuhlobo lwe-sparger, kodwa ngokubanzi ibandakanya ukucwina i-sparger kwisisombululo sokucoca, kulandelwa ukuhlamba ngokucokisekileyo ngamanzi. Ukongeza ekucoceni rhoqo, i-sparger kufuneka ihlolwe rhoqo ngomonakalo okanye ukugqoka kwaye ithathelwe indawo njengoko kuyimfuneko.

Iindidi zesparger ezisetyenziswa kwi-fermenters

1. Umsasazi wePorous:

Iiporous spargers zenziwe ngezinto ezahlukeneyo ezibandakanya isinyithi esine-sintered, i-ceramics kunye neepolymers. Ezi spagers zinendawo ephezulu kunye nenani elikhulu leepores ezincinci, ezivumela ukuba igesi isasazwe ngokulinganayo kulo lonke i-fermentation medium. Ukusebenza okuphezulu kwe-porous spagers kubenza babe lukhetho oludumileyo lokuvundiswa okukhulu, apho amazinga aphezulu okuhanjiswa kwegesi abaluleke kakhulu ekukhuleni kwe-microbial. I-porous spagers nayo inenzuzo yokuba kulula ukuyicoca kunye nokugcina, njengoko idla ngokuyilwa ukuba idibanise kwaye icocwe phakathi kweebhetshi.

2. Umdubuli wamaqamza:

Izitofu zebhamuza, ezikwabizwa ngokuba zii-diffusers, zazisa irhasi kwindawo yokubilisa ngohlobo lwamaqamza. Ezi spagers zidla ngokwenziwa ngezinto ezinentunja okanye umnatha ocolekileyo odala amaqamza amancinci njengoko igesi idlula. Izitofu zeBubble ziqhele ukusetyenziselwa ukubilisa okuncinci apho amazinga aphezulu okuhanjiswa kwerhasi engabalulekanga kangako. I-Bubble spargers ine-advanteji yokuba ilula kwaye ingabizi kakhulu ukuyisebenzisa, kodwa isenokungasebenzi kakuhle kunee-porous spagers kwaye idale isiphithiphithi ngakumbi kwindawo yokuvubela.

3. Umsasazi we-Membrane:

Iimembrane spagers zisebenzisa irhasi inwebu evuzayo ukungenisa igesi kwindawo yokubila. Ezi spagers zinendawo ephezulu kunye nenani elikhulu leepores ezincinci, ezivumela ukuba igesi isasazwe ngokulinganayo kulo lonke i-fermentation medium. Iimembrane spagers zisebenza kakuhle kwaye ziyakwazi ukudlulisa irhasi ngesantya esiphezulu, nto leyo ezenza zibe lukhetho oludumileyo lokubilisa okukhulu. I-Membrane spagers, nangona kunjalo, kunokuba nzima ukuyicoca kwaye igcinwe ngenxa yokuba i-membrane ihlala ibuthathaka kwaye yonakaliswa lula.

4. IiAerators zoMphezulu:

Ii-aerators ezingaphezulu, ezikwaziwa ngokuba zii-agitators, zidala ukuqukuqela kwesiphithiphithi kumphezulu wendlela yokubila, evumela irhasi ukuba ifunxe kulwelo. Aba basasazo badla ngokusetyenziswa kwizikali ezincinci zokubilisa apho amazinga okuhanjiswa kwerhasi ephezulu engabalulekanga kangako. Ii-aerator zomphezulu zinoncedo lokuba zilula kwaye zingabizi kakhulu ukuzisebenzisa, kodwa zisenokungasebenzi kakuhle kunezinye iindidi ze-sparger kwaye zidale isiphithiphithi ngakumbi kwindawo yokuvubela.

5. Isirinji:

Abahlohli basebenzisa iijethi zerhasi ezinoxinzelelo oluphezulu ukwenza isiphithiphithi kwi-fermentation medium, ebangela ukuba irhasi ifakwe kulwelo. Ezi zisasazo ziqhele ukusetyenziswa kwizikali ezincinci zokubilisa kwaye zinenzuzo yokuba kulula ukuyisebenzisa kwaye azibizi. Nangona kunjalo, ii-injection zinokuthi zingasebenzi kakuhle kunezinye iintlobo ze-spargers kwaye zidale isiphithiphithi kwi-fermentation medium.

Khetha isisasazo esifanelekileyo:

Xa ukhetha i-sparger ye-fermenter, kufuneka kuqwalaselwe iinkalo ezininzi, ezifana nohlobo lwezilwanyana ezincinci ezikhuliswayo, ubukhulu be-fermenter, kunye neemeko ezinqwenelekayo zokuvundiswa. Ukhetho lwe-sparger lunokuxhomekeka kwiimfuno ezithile zenkqubo yokuvutshelwa, njenge-concentration efunekayo ye-oksijeni edibeneyo okanye uluhlu lwe-pH oluphezulu. Ukugcinwa okufanelekileyo kwe-sparger kunye nokucoca kubalulekile ukuqinisekisa ukusebenza kakuhle kunye nokuthintela ungcoliseko. Ngokuqonda iintlobo ezahlukeneyo zokusasaza ezikhoyo kunye nezinto ezintle kunye nezingalunganga zazo, iingcali zokuvutshelwa zinokwenza izigqibo ezinolwazi malunga nendlela yokuphucula inkqubo yokuvutshelwa kunye nokufezekisa iziphumo ezinqwenelekayo.

Umsebenzi ophambili we-sparger kwi-fermenter

1. Ukukhutshwa kweoksijini:

Omnye wemisebenzi ephambili ye-sparger kwi-fermenter kukubonelela ngeoksijini kwii-microorganisms ezikhuliswayo. Oku kubaluleke ngakumbi kwiimicroorganisms ze-aerobic ezifuna ioksijini yokukhula kunye nemetabolism. I-sparger ihambisa ioksijini kwindawo yokuvubela ngendlela yamaqamza omoya okanye ukuhamba komoya, okwenza iintsholongwane zifunxe ioksijini kwaye ziyisebenzisele ukuvelisa amandla.

2. Hlanganisa:

Ukongeza ekuboneleleni ngeoksijini, i-sparger nayo idlala indima ebalulekileyo ekuxubeni i-fermentation medium. Xa irhasi ingeniswa kwindawo ephakathi, idala isiphithiphithi, esinceda ukusasaza izondlo kunye nezinye izithako eziyimfuneko ngokulinganayo kulo lonke ulwelo. Ukuxuba okufanelekileyo kubalulekile ukuze kuqinisekiswe ukukhula kwe-microbial okugqibeleleyo njengoko kuvumela ii-microorganisms ukuba zifumane izondlo ezizidingayo kwaye zisuse inkunkuma.

3. Ulawulo lwePH:

I-Spargers ingasetyenziselwa ukulawula i-pH ye-fermentation medium ngokuzisa i-carbon dioxide okanye ezinye iigesi ezine-asidi kulwelo. Oku kunceda ukugcina i-pH ngaphakathi koluhlu olufunwayo kwaye ithintele ukukhula kwee-microbes ezinobungozi ezikhula kwiindawo ezine-acidic.

4. Ulawulo lobushushu:

Kwezinye iimeko, i-sparger ingasetyenziselwa ukulawula ubushushu bendawo yokuvundisa. Ngokuzisa ukuhamba komoya opholileyo, i-sparger inokunceda ukunciphisa ubushushu beendaba kunye nokukhusela ukushisa. Ngokwahlukileyo, umoya oshushu ungasetyenziselwa ukwandisa ubushushu bendawo ephakathi kunye nokukhuthaza ukukhula kwe-microbial.

5. Ukulawula amagwebu:

Okokugqibela, izitshizi zisenokusetyenziswa ukulawula ukumila kwegwebu ngexesha lokubila. I-Foam ingaba yingxaki kwi-fermentation enkulu njengoko iphazamisa inkqubo yokuxuba kunye nokunciphisa ukusebenza kakuhle komthengisi. Ngokuzisa ukuhamba komoya kwindawo yokuvubela, i-spargers inokunceda ukuqhekeza ugwebu kwaye ithintele ukwakheka kwegwebu.

ukuququmbela:

Isishwankathelo, i-spargers idlala indima ephambili kwinkqubo yokuvutshelwa ngokubonelela nge-oksijini, ukuxuba imidiya ye-fermentation, ukulawula i-pH kunye nokushisa, kunye nokuthintela ukubunjwa kwe-foam. Ukukhetha uhlobo oluchanekileyo lwe-sparger kunye nokugcina kunye nokucoca ngokufanelekileyo kubalulekile ekuphuculeni inkqubo yokuvutshelwa kunye nokufezekisa iziphumo ezifunwayo. Ngokuqonda umsebenzi womthengisi kwi-fermenter, iingcali zokuvutshelwa zinokwenza izigqibo ezinolwazi malunga nendlela yokuphucula inkqubo yokuvutshelwa kunye nokufezekisa iziphumo ezifunwayo.

Ukusetyenziswa komthengisi kwi-fermenter

1. Ukuveliswa kwebhiya newayini:

Enye yezona zicelo zixhaphakileyo kwizisasazo kwizibilisi kukuveliswa kwebhiya newayini. Kula mashishini, i-spargers isetyenziswa ngokuqhelekileyo ukubonelela i-oksijini kwigwele ngexesha lokuvutshelwa, okunceda ukukhuthaza ukukhula kwegwele okunempilo kunye nokuvuthwa okusebenzayo. I-Spargers ingasetyenziselwa ukunceda ukulawula ubushushu kunye ne-pH ye-fermentation medium, ezibaluleke kakhulu ekufezekiseni incasa efunwayo kunye nenqanaba lotywala kwimveliso yokugqibela.

2. Bioremediation:

I-Spargers iphinda isetyenziswe kwintsimi ye-bioremediation, ebandakanya ukusetyenziswa kwe-microorganisms ukudiliza izinto ezingcolileyo kunye nezinye izinto ezingcolileyo kwindalo. Kule sicelo, i-spargers isetyenziselwa ukwazisa i-oksijini kwindawo engcolileyo, enceda ukukhuthaza ukukhula kwee-microorganisms ezijongene nokudiliza izinto ezingcolileyo. Le nkqubo isebenzayo ngokukodwa ekucoceni umhlaba kunye namanzi angaphantsi komhlaba angcoliswe yimveliso yepetroleum, i-solvents kunye nezinye iikhemikhali zoshishino.

3. Imveliso yamayeza:

Abasasazi basetyenziswa ngokubanzi kwishishini lamachiza ukuvelisa izitofu zokugonya, ii-antibiotics kunye namanye amayeza. Kule zicelo, i-spargers isetyenziselwa ukwazisa i-oksijini kwiinkcubeko ze-microbial ezijongene nokuvelisa imveliso efunwayo. I-Spargers inokunceda kwakhona ukuxuba i-fermentation medium kunye nokulawula ubushushu kunye ne-pH yenkcubeko, ebaluleke kakhulu ekugcineni impilo kunye nemveliso yeenkcubeko ze-microbial.

4. Ukuvubela kwimizi-mveliso:

IiSparger zikwasetyenziswa ngokuqhelekileyo kubiliswa kwimizi-mveliso emikhulu ukuvelisa iienzymes, iiamino acids, kunye nezinye iimveliso zebhayoloji. Kwezi zicelo, i-sparger isetyenziselwa ukubonelela nge-oksijini kwinkcubeko ye-microbial kunye nokunceda ukugcina ubushushu kunye ne-pH efunwa yinkcubeko. Ukuhluthwa okufanelekileyo kubalulekile ekufezekiseni izivuno eziphezulu kunye nokugcina impilo kunye nemveliso yeenkcubeko ze-microbial.

5. UPhando noPhuhliso:

Okokugqibela, abasasazo bahlala besetyenziselwa uphando kunye nophuhliso lwezicwangciso zokufunda ukukhula kunye nokuziphatha kwee-microorganisms kwindawo elawulwa yilabhoratri. I-Spargers ingasetyenziselwa ukwazisa iindidi zegesi kwimidiya yokuvutshelwa, evumela abaphandi ukuba bafunde imiphumo yeemeko ezahlukeneyo zokusingqongileyo ekukhuleni kwe-microbial kunye ne-metabolism.

ukuququmbela

Isishwankathelo, i-spargers ziyinxalenye ebalulekileyo yeenkqubo ezininzi zokuvutshelwa, ukubonelela nge-oksijini, ukuxuba imidiya ye-fermentation, kunye nokulawula ukushisa kunye ne-pH yenkcubeko. Ukusetyenziswa kwe-spreaders kubalulekile ekufezekiseni izivuno eziphezulu kunye nokugcina impilo kunye nemveliso yeenkcubeko ze-microbial. Ngokuqonda usetyenziso olwahlukeneyo kunye nokusetyenziswa kwezisasazo ekuvubeleni, iingcali zokuvutshelwa zinokwenza izigqibo ezinolwazi malunga nendlela yokuphucula inkqubo yokuvubela kunye nokufikelela kwiziphumo ezinqwenelekayo.

Sesiphi isitshizi esidumileyo seSivundiso?

nozzle sprayer kwi fermenter kunyeAbasasazi be-Orifice kwizibilisi

Imilomo kunye ne-orifice abasasazi ziindidi ezimbini ezidumileyo zabasasazi ezisetyenziswa kwizibilisi. Nalu ulwazi oluninzi malunga nenye:

1. Abasasazi bemibhobho kwizivundisi:

I-nozzle sparger yi sparger esebenzisa uthotho lwemilomo ukungenisa amaqamza omoya kwindawo yokubila. Imilomo idla ngokucwangciswa ngokwepateni yegridi emazantsi esixhobo sokuvundisa apho irhasi iqaliswa ngesantya esilawulwayo. I-Nozzle spagers zithandwa kakhulu kwi-industrial-scale fermentation kuba zibonelela ngokuxutywa okusebenzayo kunye ne-oxygen ye-fermentation medium. Kwakhona kulula ukuzicoca nokuzilungisa.

2. Abasasazi be-Orifice kwizibilisi:

I-orifice sparger yi-sparger esebenzisa uthotho lwemingxuma emincinci okanye i-orifices ukwazisa amaqamza omoya kwindawo yokuvubela. Ii-orifices zivame ukucwangciswa kwipatheni yegridi ezantsi kwe-fermenter apho igesi ifakwe kwizinga elilawulwayo. I-Orifice spargers ithandwa kwiindawo ze-R & D ngenxa yokuba ivumela ulawulo oluchanekileyo lwamazinga okungenisa igesi kwaye ingasetyenziselwa ukufunda imiphumo yeendlela ezahlukeneyo zegesi ekukhuleni kwe-microbial kunye ne-metabolism.

Zombini imilomo kunye ne-orifice spargers ziyasebenza ekuboneleleni ngeoksijini kwinkcubeko ye-microbial kunye nokuxuba i-fermentation medium. Ukukhethwa kwe-sparger kuxhomekeke kwiimfuno ezithile zenkqubo yokuvundiswa, kubandakanywa nobukhulu be-fermenter, uhlobo lwe-microorganisms ekhuliswayo, kunye nesiphumo esifunwayo sokuvutshelwa. Ekugqibeleni, injongo kukukhetha i-sparger enika ukuhanjiswa kwegesi ngokufanelekileyo kwaye ikhuthaze ukukhula kwe-microbial enempilo ngelixa unciphisa ukubunjwa kwe-foam kunye nezinye iziphumo ezingathandekiyo.

ukuququmbela:

Isishwankathelo, i-sparger yinxalenye ebalulekileyo yenkqubo yokuvutshelwa kwaye idlala indima ephambili ekulawuleni ukuxinwa kwe-oksijeni echithwe kunye ne-pH kwindawo yokuvuthela. Ukukhetha uhlobo oluchanekileyo lwe-spreader kunye nokugcina kunye nokucoca ngokufanelekileyo kunokunceda ekuqinisekiseni inkqubo yokuvutshelwa elungiselelwe ubuninzi besivuno kunye nomgangatho. Ngokuqonda ukuba yintoni isisasazo kunye nendlela esisebenza ngayo, iingcali zokuvutshelwa zinokwenza izigqibo ezinolwazi malunga nendlela yokuphucula inkqubo yokuvutshelwa kunye nokufezekisa iziphumo ezinqwenelekayo.

Ngaba unomdla wokuphucula inkqubo yakho yokuvubela kunye nezisasazo?

Ukuba kunjalo, ungathandabuzi ukujonga iindlela ezahlukeneyo zokusasaza ezikhoyo ukuze ubone ukuba banokuzuza njani kwisicelo sakho esithile.

Qhagamshelana nengcali yokuvutshelwa kwaye ukhethe okanye i-OEM ekunene sintered sparger namhlanje ukufunda ngakumbi kwaye uqalise ukuphucula inkqubo yakho yokubila!

Izibonelelo ezongezelelweyo:

Kubafundi abanomdla wokufunda ngakumbi malunga nezisasazo kwi-fermenters, izixhobo ezininzi ziyafumaneka. Ezinye izixhobo ezicetyiswayo ziquka:

I-"Fermentation Microbiology and Biotechnology" yi-EMT El-Mansi, iCFL Amaral kunye neWWM Souza (2019)
"Imigaqo ye-Bioprocess Engineering" nguPauline M. Doran (2012)
"Incwadana yeNkcubeko yeeSeli zeShishini: iMammalian, iMicrobial, kunye neeSeli zeZityalo" uMhleli oyiNtloko uVictor G. Aunin (2010)